Journal: PLoS ONE

Article Title: Investigations on the mode of action of gephyronic acid, an inhibitor of eukaryotic protein translation from myxobacteria

doi: 10.1371/journal.pone.0201605

Figure Lengend Snippet: KB-3-1 cell lysates treated with varying concentration of GA were subjected to DARTS analysis with pronase. Lysates treated with cycloheximide served as negative control. GA binding protected the initiation factor eIF2α in a dose dependent manner. GAPDH was used as a loading control.

Article Snippet: The membrane was blocked with 5% BSA and probed with rabbit polyclonal antibodies against eIF2α and GAPDH (Cell Signalling).

Techniques: Concentration Assay, Negative Control, Binding Assay, Control

Journal: PLoS ONE

Article Title: Investigations on the mode of action of gephyronic acid, an inhibitor of eukaryotic protein translation from myxobacteria

doi: 10.1371/journal.pone.0201605

Figure Lengend Snippet: PtK2 cells were treated with 50 nM of GA (upper row) or biotinylated GA methyl ester (lower row) for three hours and stained for eIF2α (red) and biotin (green). The green biotin label often co-localizes with the red one of eIF2α shown by the resulting yellow color in the overlay image (bottom left).

Article Snippet: The membrane was blocked with 5% BSA and probed with rabbit polyclonal antibodies against eIF2α and GAPDH (Cell Signalling).

Techniques: Staining

Journal: PLoS ONE

Article Title: Investigations on the mode of action of gephyronic acid, an inhibitor of eukaryotic protein translation from myxobacteria

doi: 10.1371/journal.pone.0201605

Figure Lengend Snippet: KB-3-1 cell lysates were incubated with methanol, GA or biotinylated GA methyl ester and the targets pulled down using a streptavidin column. The eluates were analyzed by western blotting. The samples from the lysates treated with C3-biotinylated GA methyl ester showed clear bands of eIF2α at 36 kDa that were almost not present in the preparations with methanol or GA only. Biotin containing pyruvate carboxylase, which is also fished, served as a control.

Article Snippet: The membrane was blocked with 5% BSA and probed with rabbit polyclonal antibodies against eIF2α and GAPDH (Cell Signalling).

Techniques: Incubation, Western Blot, Control

Journal: Experimental hematology

Article Title: Panobinostat and venetoclax enhance the cytotoxicity of gemcitabine, busulfan, and melphalan in multiple myeloma cells

doi: 10.1016/j.exphem.2020.01.003

Figure Lengend Snippet: List of primary antibodies, their sources and dilutions

Article Snippet: eIF2α , Cell Signaling/9722 , Rabbit , 2500.

Techniques: